Coexistence of Aldose Reductase Gene and Endothelial Nitric Oxide Synthase Polymorphisms Associates with Diabetic Nephropathy
摘要
BACKGROUND: It has been demonstrated that the C-106T polymorphism at promotor region of aldose reductase gene and GLU298ASP (894G→T) polymorphism at exon 7 of endothelial nitric oxide synthase (eNOS) gene are associated with diabetic nephropathy, it should be further studied whether the risk for diabetic nephropathy will increase when the above polymorphic sites coexist. OBJECTIVE: To investigate the association between the coexistence of the polymorphisms of both the C-106T at Fu promotor region of aldose reductase gene and GLU298ASP (894G→T) at exon 7 of eNOS gene in chromosome 7q35 region and the genesis of diabetic nephropathy in northern Chinese Han people. DESIGN: A case-controlled, comparative observation. SETTING: Department of Endocrinology, the Affiliated Hospital of Medical College, Qingdao University. PARTICIPANTS: Totally 139 inpatients with type 2 diabetes mellitus were selected from the Department of Endocrinology, the Affiliated Hospital of Medical College, Qingdao University from November 2002 to April 2005, including 54 males and 85 females, (64±8) years of age. Inclusive criteria: Accorded with the standards of diabetic diagnosis and classification by WHO in 1999. According to the 24-hour urinary albumin excretion rate (UAER), they were divided into diabetic nephropathy group (n=61) and non-diabetic nephropathy group (n=78). Meanwhile, 63 healthy controls were selected as the control group, including 24 males and 39 females, 50-78 years of age. All the enrolled subjects were Han people. Informed contents were obtained from all the subjects. METHODS: The genotypes and alleles of polymorphisms of GLU298ASP(894G→T) at exon 7 of eNOS gene and C-106T at promotor region of aldose reductase gene were detected by polymerase chain reaction restriction-fragment length polymorphism technique (PCR-RFLP), DNA sequencing technique and agarose gel electrophoresis separation technique. Then the frequency of genotypes and alleles were compared. MAIN OUTCOME