Production of chitinolytic enzymes with Trichoderma longibrachiatum IMI 92027 in solid substrate fermentation
摘要
Thirty Trichoderna strains representing 15 species within the genus have been screened for extracellular production of chitinolytic enzymes in solid substrate fermentation (SSF). T.longibrachiatum IMI 92027 ( = ATCC 36838) gave the highest yield (5.0 IU/g dry matter of substrate) after 3 days of fermentation on wheat bran-crude chitin (9:1 mixture) medium. The optimum moisture content (66.7 %), chitin content (20 %), initial pH of the medium (2-5) and time course (5 d) of SSF were determined for strain IMI 92027. No significant effect of different N and P additives was found on the chitinase yield in wheat bran-chitin mixture medium.
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KrisztinaKovacs, GyorgySzakacs, TundePusztahelyi, 等. Production of chitinolytic enzymes with Trichoderma longibrachiatum IMI 92027 in solid substrate fermentation[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2004.
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