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Expression of human clotting factor IX with EBV shuttle vector

王洪伟、郑冰、薛京伦、邱信芳

1995中国科学通报：英文版Biochemistry, Genetics and Molecular Biology被引 5

摘要

Culture of patient somatic cells, transfected with therapeutic genes into genomic DNA with retroviral vectors, followed by colony selection, amplification, and reimplantation of transformed cells into patient, has been Widely used for clinical trial of gene therapy in the past years. But the disadvantages of this protocol are obvious. (i) To a great extent the expression level of the transfected cells depends on the different integration sites,which cause the various expression rates from different colonies; besides, the life span of

引用本文（GB/T 7714）