EFFECTS OF XW630 ON BONE FORMATION IN OVARIECTOMIZED RATS AND IN HUMAN OSTEOBLAST-LIKE CELLS
摘要
Objective. To study the effects of XW630 on bone formation in overiectomized(OVX) rats and in hu-man osteoblast-like cell line TE85. Method. Bone histomorphometric analysis was performed with undecalcified bone sections and tetracy-cline intraperitoneally labeling. Results. Compared with that of OVX rats, the static data of trabecular bone volume(TBV)/total tis-sue volume(TTV), TBV/sponge bone volume(SBV) and mean trabecular plate density (MTPD) were en-hanced while mean trabecular plate spacing(MTPS) decreased after treated with XW630 for 13w. The dy-namic data of single-labeled surface [Sfract(s)], double-labeled surface[Sfract(d)],Sfract(d+1/2s),tra-becular osteoid surface(TOS), and bone formation rate in tissue level (Svf) were increased and osteoid maturation period (OMP) shortened in XW630 group. In osteoblast-like cells, both ^3H-thymidine incorpo-ration and cell count increased after treated with XW630 for 48. Treated with XW630 for 12～ 18h,in-ducible nitric oxide synthase(iNOS) activity and cGMP content increased in time-dependent manners. Conclusions. XW630 enhanced bone activation frequency and increased trabecular connectivity, stabili-ty, and strength. The cellular mechanism related to effects of XW630 on bone formation in ovariectomized