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大孔吸附树脂在瑞拉菌素高产菌GAO-1-GR-2活性物质提取中的应用

程建坤、侯冬媛、苟丽霞、安德荣

2010Biochemistry, Genetics and Molecular Biology被引 0

摘要

Based on fermentation broth of Zuelacmycin-high-yielding strain GAO-1-GR-2, antifungal active substances were extracted from the fermentation broth on the conditions of different extracting temperatures and five different kinds of macroporous adsorption resins. Phyricularia grisea was used as indicating microorganism. The results showed that the optimum extracting temperature was 60℃ and macroporous adsorption resin HP-20 had better adsorption effect to active substances. After the optimization of absorption conditions, the optimum pH of sample was 6.0, optimum absorption velocity was 2.0mL/min, the optimum component of cluent was methanol 50%, ethanol 70% and acetone 30% (by vol), the optimum velocity of cluent was 1.5mL/min

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