中国对虾(Fenneropenaeus chinensis)“黄海1号”部分生长相关性状的QTL定位分析
摘要
The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp F. chinensis was captured from the west coast of the Korean peninsula and mated with a ”Yellow Sea No.1” male to produce the F1. F2 families with a sum of 100 individuals from the brother-sister cross among the F1 families. In the present study, a genetic linkage map of the Chinese shrimp was constructed, based on 354 markers including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers and 12 randomly amplified polymorphic (RAPD) markers. Forty-seven linkage groups (LGs) were found. The total map length was 4580.5cM, with an average spacing of 11.3cM, covering 75.8% of the estimated genome size. Based on the constructed linkage map using the Chinese shrimp F2 population, marker regression and complexity interval mapping were analyzed with the WinQTLcart 2.5 software. A linkage group-wide permutation test (1000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group. On the study of QTL mapping among body weight (BW), abdominal segment length (AL) and telson length (TL), a total of 29 QTLs were located, nine significant QTLs (BW1.2, A1L2.1, A1L9.2, A1L17.3, A1L26.4, A3L16.1, A4L26.1 and T3L17.3) were at the 5% linkage group-wide level for these linkage groups, and A3L16.1 for abdominal segment length was at 1% level. The variances explained by these QTLs ranged from 11.14% to 23.60%; and their additive effects were not identical. Except A1L2.1 and A6L20.3, the other seven major QTLs were positive. In addition, some molecular markers (D9f446, SX18, M10f24 and B2f260) were tightly linked to their corresponding traits, which could be used for fine mapping of these QTLs.