中国部分汉族人群中IL-6基因启动子-572G／C多态性与HBV感染易感性的关系
摘要
AIM: To evaluate the association of IL-6 gene promoter polymorphism with the infection of hepatitis B virus (HBV) in Chinese population. METHODS: The genome DNA was obtained from blood samples and three IL-6 promoter polymorphisms (G-174C, G-572C and G-597A) were evaluated using PCR-RFLP in 160 HBV-infected patients and 212 healthy controls. Frequencies of single-nucleotide polymorphisms (SNPs) in the control group were compared with those of the HBV infection. Furthermore, subgroups of chronic carriers were compared among them. For cases and controls, the distribution of gender, age, smoking, drinking and Hardy-Weinberg equilibrium equation of the IL-6 genotype was determined using Chi-square Test; and the diversity of age using Mann-Whitney U-test. The unconditional Logistic regression model was used to analyze the statistical association of genotypes in two groups adjusted for confused factors. RESULTS: No polymorphism at position -174 and -597 was detected in all participants, but significant difference was found in the polymorphism at position -572 between the cases and controls (G/C vs G/G, OR=2.65, P＜0.05; C/C vs G/G, OR=3.31, P＜0.05). Subgroups analysis, revealed the odds ratio value in the less than thirty years old group (G/G vs G/C or C/C, OR=16.92, P＜0.05). CONCLUSION: The data reveal that the polymorphism at position -572 of the IL-6 gene promoter may be associated with the susceptibility to HBV infection; meanwhile, the age is a coordinative risk factor.