载脂蛋白A5基因－1131T/C多态性与2型糖尿病血脂及其颈动脉内膜中层厚度相关性研究
摘要
Objective To investigate the relationship between apolipoprotein A5 gene-1131T/C polymorphism and serum lipids levels and carotid intima-media thickness in patients with type 2 diabetes mellitus in a Chinese population in Chengdu. Methods The genotype and allele frequencies of apolipoprotein A5-1131T/C polymorphism were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide electrophoresis (PAGE) methods. The serum lipids were measured with enzymatic kits in 154 type 2 diabetic patients and 206 normal people (control). The carotid intima-media thickness (IMT) of 116 type 2 diabetic patients was measured by B-mode ultrasonography. Results The allele frequencies of T, C in the diabetic patients were 0.721, 0.279, respectively, which were not significantly different from those of the normal controls (0.760, 0.240). Compared with the wild type TT, CC homozygote increased the risk of type 2 diabetes significantly (OR=2.432, 95%CI: 1.081-5.473). In the patients with type 2 diabetes, the serum triglyceride (TG) level and TG/HDL-C ratio were greater in those with TC and CC genotypes than those with TT genotype subjects (P＜0.05). The normal people with TC genotype also had greater triglyceride levels and TG/HDL-C ratio than those with TT genotype. The diabetic patients with CC genotype had greater carotid IMT than those with TT genotype (P=0.08). Conclusion The-1131T/C polymorphism in the apolipoprotein A5 gene may have an impact on serum triglyceride levels and TG/HDL-C ratio. People with homozygote CC have increased risk of type 2 diabetes. But more evidence is needed to prove its association with carotid IMT in patients with type 2 diabetes.