CIK/NK细胞4周培养过程中的CD27、CD28的表达及其意义
摘要
AIM: To investigate the changes of CD27 and CD28 expression on cytokine-induced killer (CIK) cells and natural killer (NK) cells during cultivation. METHODS: The mononuclear cells were treated with interleukin-12 (IL-2), IL-7, IL-15, stem cell factor and FLT3 ligand for four weeks. The CD27 and CD28 expression on CD3(superscript +) CD56(superscript +) CIK cells and CD3(superscript -) CD56(superscript +) NK cells were examined by flow cytometric analysis every week during four-week cultivation. RESULTS: The expression of CD27 on CIK and NK cells two weeks after cultivation reached the peak value at (44.57±4.07) % and (51.02±5.20)%, respectively. Then, their expression declined gradually to about 30% in the fourth week. The expression of CD28 on CIK and NK cells reached the peak at the third and the second week, the values were (4.40±0.66)% and (45.14±3.58)%, respectively, decreased rapidly and then were almost completely lost at the fourth week. CONCLUSION: According to the changes of expressions of CD27 and CD28 on CIK/NK cells, optimal harvest time of cultured CIK/NK cells should be at the third week of cultivation.