转录因子T-bet/GATA-3诱导哮喘模型中α-SMA和I型胶原高表达的实验研究
摘要
Objective To study the relationship between transcription factor T-bet/GATA-3 and expression of α-smooth muscle actin (α-SMA) and type Ⅰ collagen in asthma. Methods The levels of T-bet and GATA-3 in rat lungs were determined by quantitative real-time PCR and western blotting respectively. α-SMA and type Ⅰ collagen levels in lung fibroblasts were determined by immunohistochemistry and ELISA respectively. Results The expression of T-bet mRNA and protein in the OVA group was significantly attenuated compared with that in the saline group and the blank group, while the expression of GATA-3 in the OVA group was significantly increased and the T-bet/GATA-3 ratio in the OVA group was significantly decreased. The α-SMA and type Ⅰ collagen levels in the OVA group were significantly increased. The T-bet/GATA-3 ratio had a negative correlation with α-SMA and type Ⅰ collagen. There was no significant difference between the saline group and the blank group. Conclusion The T-bet/GATA3 ratio has a negative relation to α-SMA and type Ⅰ collagen, and T-bet/GATA3 imbalance plays a crucial role in asthma models.