Down-regulation of γ-synuclein in human esophageal squamous cell carcinoma
摘要
AIM: To investigate gene expression pattern of human γsynuclein gene in human esophageal squamous cell carcinoma (ESCC) by using semi-quantitive reverse transcription polymerase chain reaction (RT-PCR), and to study the role of γ-synudein in the development of human ESCC.METHODS: Semi-quantitive RT-PCR of 27 pairs of specimens of human ESCC tissues and corresponding normal tissues was used to investigate the expression pattern of γsynuclein in ESCC. 9706/γ-syn cells in which γ-synuclein was overexpressed were obtained through cloning γ-synuclein gene by PCR and transfecting it into ESCC 9706 cells, then selecting with G-418 for 14 days. The biological effects of γsynuclein were measured and compared between 9706/γ-syn and 9706/vec cells by cell growth curve and soft agar assay.RESULTS: RT-PCR showed that γ-synuclein gene was expressed in all the 27 cases of normal epithelial tissues,while downregulation of γ-synudein was observed in 16 out of the 27 cases (59.3%) of ESCC. There were also 6 cases of ESCC tissues with a high expression level of γ-synuclein mRNA. In functional analysis we found that over-expression of γ-synuclein in ESCC 9706 cells could inhibit the growth rate and transformation ability of ESCC 9706 cells.CONCLUSION: The low expression level of γ-synuclein in human ESCC and the biological effects of γ-synuclein overexpression on ESCC 9706 cells suggest that γ-synuclein may play a role as a negative regulator in the development of human ESCC.