TGF-β1对Burkitt淋巴瘤细胞增殖及c-myc表达的影响
摘要
OBJECTIVE: To investigate the inhibition of TGF-β1 on cell growth, c-myc gene and protein expression in Burkitt lymphoma cell line. METHODS: Five ng/mL TGF-β1 in Burkitt lymphoma cell line was added in the experimental group, and having no TGF-β1 in the same cell line in the control group. The two groups were cultured for 24, 48 and 72h, respectively, and the cell survival rate, c-myc mRNA and protein expression were detected by using MTT, RT-PCR and Western Blotting analysis methods. RESULTS: The Jiyoye cell survival rates in the experimental group were (80.5±3.14)%, (70.5±3.77)% and (56.4±3.360% at 24, 48 and 72h, respectively. Compared with the control group, the cell survival rates were significantly lower in experimental group (t=8.7880, P=0.0127; t=13.5709, P=0.0054; t=22.5006, P=0.0197). The survival rate at 72h was also significantly lower than that at 24h in the experimental group (t=8.1862, P=0.0012). c-myc mRNA and protein expression began to reduce at 24h, and in evidence inhibited at 48h and 72h, and this was significantly lower than that in the control group, P=0.0000. CONCLUSIONS: The inhibition of TGF-β1 on Jiyoye cell gowth is dependent on the time process. The mechanism might be related with the inhibition of c-myc gene and protein expression.