双歧杆菌总DNA对小鼠IL-2、NK活性影响的研究
摘要
Objective Our purpose was to get Out DNA from Bifidobacteria and E. coli, using the DNA to immune mice; observing the change of immunological function; discussing the immunization of Bifidobacteria DNA and constrasting the research. Methods Mice of Bifidobacteria DNA and E. coli DNA-treated groups were respectively injected with DNA by muscle, then by subcutaneous. We detected he immunological function of spleen cell, got out IEL cell and incubated them with DNA, detected TEL activity and the production of cytokine. Results IL-2 and NK activity were all signicantly higher than those in control group (P＜0.01). IL-2 and NK activity of Befidobacteria DNA group were higher than those of E. coli DNA group (P＜0.01). Conclusion The bifidobacteria DNA and the E. coli DNA could regulate mocosal immunity by enlarging natural killing activity and secreting cytokine as JL-2. The bifidobacteria DNA was better than the E. coli DNA.