Surveillance of Kanamycin Resistance to Escherichia coli from Swine by Digoxigenin-labled Plasmid Probe
摘要
A 4.34kb EcoR I fragment of kanamycin resistance plasmid from pET - 9a was purified by a DNA purification kit. The fragment was labeled with digoxigenin-dUTP with a commercial kit. A dot-blot hybridization and a colony hybridization test with the probe were successfully developed for the surveillance of Kanamycin resistance to E. coli from swine. It was shown that the methods obtained 100% concordance in a positive tate. It was indicated that the method was available for the surveillance of kanamycin resistance to E.coli from swine.
引用本文（GB/T 7714）
Yang, Han-chun, Zhao, 等. Surveillance of Kanamycin Resistance to Escherichia coli from Swine by Digoxigenin-labled Plasmid Probe[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2002.
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