斜纹夜蛾核型多角体病毒日本株（C3）ie0基因的序列分析和表达
摘要
The ie0 gene was cloned from the genome of Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) JP-C3 which was isolated in Japan, Nucleotide sequence analysis demonstrated that this clone included 864 bp open reading frame(ORF), 475 hp of 5'-end promoter region and 90 bp of 3'-end terminator region. Amino acid sequence analysis showed that there were abundant acidic and hydrophobic amino acid residuals at the 23th to 47th sites and the 111th to 126th sites in N-terminus side of Sp1tMNPV IE0, a typical double zinc-finger structure at 225th to 271th sites in C-terminus side, which was conservative in IE0 of the nine comparative insect baculoviruses. On-line analysis indicated that nucleotide sequence and amino acid sequence of SpltMNPV JP-C3 were distinct from those of the other NPVs. The phylogenetic tree of baculoviruses based on the ie0 gene incorporated BmNPV (Bombyx mori Nucleopolyhedrovirus) and AcMNPV(Autographa californica multicapcid nucleopolyhedrovirus) into the same branch, which was identical to those based on p10, p37 and e genes, and expressed in E. coli BL21, identified by Western Blotting.