COX-2、Ki-67和结核菌L型感染在前列腺癌中的表达
摘要
Objective To explore Cox-2 and Ki-67 in prostate carcinoma as well as L-type Mycobacterium tuberculosis infection and its clinical significance. Method Immunohistochemistry, in situ hybridization and other detection methods such as acid-fast stain were applied to 65 cases of prostate cancer (carcinoma of prostate, PCa) and 30 cases of benign prostatic hyperplasia (benign prostatic hyperplasia, BPH) for the expressions of Cox-2, Ki-67 protein and mRNA, as well as the detection rate of TB L-type. The main clinical data and pathological classification parameters of prostate cancer were analyzed statistically with χ^2 test. Result The expressions of Cox-2 and Ki-67 proteins and mRNA as well as the L-TB detection rate in prostate cancer was significantly higher than those in benign prostatic hyperplasia (P＜0.001-0.05). The expressions of Cox-2 and Ki-67 proteins and mRNA as well as L-TB detection rates in prostate cancer at different clinical stages, histological grades were significantly different (P＜0.01-0.05). The Lymph node metastasis group showed significantly higher positive expression rate of cox-2 and ki-67 proteins and mRNA than the non-metastasis group did (P＜0.01). L-TB detection rate of lymph node metastasis group was significantly higher than that of non-metastasis group (P＜0.05). Conclusion Cox-2 and Ki-67 proteins and the abnormal expression of mRNA in prostate tumors as well as the L-TB detection rate are positively correlated with clinical tumor stage, grade and metastasis, suggesting that the two genes can be used as the reference indicators of biological behavior and prognosis of patients with prostate cancer. L-TB infection very likely leads to gene mutation or overexpression, and become one of the inducing factors of tumors; they may have synergistic tumorigenic effect.