E-cadherin, β-catenin, Cyclin D1在胃腺癌中的表达及临床意义
摘要
AIM: To investigate the expression of E-cadherin, β-catenin and Cyclin D1 and their relationships and clinical pathological features in gastric adenocarcinoma. METHODS: Immunohistochemical SP method was used to determine the expression of E-cadherin, β-catenin and Cyclin D1 protein in 73 cases of gastric adenocarcinoma tissues and 18 cases of normal gastric mucosal tissues. RESULTS: Positive staining for E-cadherin and β-catenin was observed on the cellular membrane of epithelial cells in normal gastric mucosal tissues. The aberrant expression of E-cadherin and β-catenin were observed in the cytoplasm and/or cellular nucleus of gastric cancer cells. The aberrant expression rates of E-cadherin and β-catenin were 63.01%(46/73) and 56.16%(41/73) in gastric adenocarcinoma, respectively, which were associated with the differentiation degree, TNM stages, depth of infiltration and lymph node metastasis, but not with the gender, age and tumor size. There was a significant correlation between E-cadherin and β-catenin expression in gastric adenocarcinoma. The positive rate of Cyclin D1 expression was 67.12%(49/73) in gastric adenocarcinoma, notably higher than that in normal gastric tissues (0/18, P＜0.01), and it was correlated with the differentiation degree and lymph node metastasis. The aberrant expression of E-cadherin and β-catenin were positively correlated with the high expression of Cyclin D1 gastric carcinoma (r=0.249, 0.376, both P＜0.05). CONCLUSION: Down-regulated expression of E-cadherin and β-catenin exist in gastric carcinoma, which may promote the pathogenesis and development of carcinoma by activation of Cyclin D1.