芒苞草(芒苞草科)种子的无菌萌发研究
摘要
Seeds of Acanthochlamys bracteata were applied to detect the effects of sterile water, liquid and solid MS media, and different kinds and concentrations of hormone on germination rate, and growth of seedlings was also studied. The results showed that the germination rate by seeds of A. bracteata cultured in sterile water could reach 95% after jarovization for 2 months at 4 ℃, much higher than those in liquid (25.1%) and solid (10.4%) MS media; 6-BA and GA3 could not promote the seed germination in sterile water, but 1.0 mg/L GA3 could hasten the germination rate in liquid MS medium and 0.5 mg/L 6-BA could promote the rate in solid MS medium as well. Furthermore, in solid MS medium fasciculate seedlings could be observed after 3 months, and in solid MS medium with 1.0 mg/L 6-BA the fasciculate seedlings were more than those with 0.5 mg/L 6-BA. The germinated seeds of A. bracteata in sterile water and liquid MS medium were transplanted into solid MS medium, and buds could grow up into seedlings after 3 months in the media with hormone at different concentrations.