细胞质唾液酸酶(Neu2)通过Cdk2通路抑制FBJ-LL细胞生长
摘要
Objective To verify the roles of Neu2 (neuraminidase) as a growth regulator in FBJ-LL cells. Methods The mRNA expression of Neu2 and Cdk2 were determined by reverse transcription-polymerase chain reaction. The sialidase activity was determined by using 4-MUNeuAC as a substrate. Cell viability was detected by tetrazolium assay. Cell cycle analysis was performed by propidium iodide staining followed by flow cytometry. Results Neu2 sense cDNA transfection significantly enhanced the mRNA expression of Neu2 and the total sialidase activity in FBJ-LL cells. Neu2 over-expression in FBJ-LL cells inhibited the cell growth and decreased the number of cells in G1 phase. Neu2 sense cDNA transfected cells showed impaired expression of Cdk2 mRNA. Conclusions Neu2 is responsible for cell growth inhibition of FBJ-LL cells through Cdk2 signal pathways.