贝科能对心肺复苏后大鼠心、脑、肾的保护作用
摘要
Objective: To observe the protective effects of Biocoen on cellular ultrastructure of heart, brain and kidney after cardiopulmonary resuscitation (CPR) in rats. Methods: Cardiac arrest was induced by asphyxiation and ice-cold 0.5 mol/L KCl; resuscitation was started 5 mm after arrest. Twenty-four Sprague-Dawley rats were randomly divided into 3 groups: sham control group, conventional treatment group and Biocoen intervention group (n=8). Using the colorimetry, we analyzed the quantity of maleic dialdebyde (MDA) and enzyme activity of superoxide dismutase (SOD) and Na(superscript +)-K(superscript +)-ATPase in the heart, brain and kidney tissues in all groups. The myocardial cell, cerebral neuron and renal tubule epithelium were observed by transmission electron microscope (TEM). Results: Compared with control group, quantity of MDA increased greatly (P＜0.05), while activity of SOD and Na(superscript +)-K(superscript +)-ATPase in heart, brain and kidney declined markedly in conventional treatment group (P＜0.05). The injury of myocardial cell ultrastructure, cerebral neuron and renal tubule epithelium, such as mitochondria swelling, cristae rupture, vacuole denaturation, was observed in conventional treatment group and Biocoen intervention group, but the injury of the Biocoen group was greatly alleviated. Conclusion: Biocoen plays an important role in the protection of cellular ultrastructure of heart, brain and kidney in rats after CPR.