三七皂苷R1诱导HL-60细胞凋亡中survivin、bcl-2的表达
摘要
[Objective] To explore Sanqi glucoside R1 function and mechanism to humanbeing leukemia cells HL-60 perishing. [Method] Under microscope observe HL-60 cell forms functioned by Sanqi glucoside, apply MTT colormetry to observe Sanqi glucoside's inhibition on HL-60 cell hyperplasis. Use flow cell instrument to measure cell perishing, RT-PCR to test expression of survivin and bcl-2. [Result] Sanqi glucoside R1 75-1200μg•ml^(-1) could inhibit humanbeing leukemia cell HL-60 production, in dependence of time and concentration. After functioned by the Sanqi glucoside R1, the humanbeing leukemia cell HL-60 was preshing; the flow cell instrument showed risen perishing cell ratio, which rose with dosage. RT-PCR showed under the function of Sanqi glucoside R1, the expression of survivin and bcl-2 was reduced. [Conclusion] Sanqi glucoside R1 can induce the perishment of humanbeing leukemia cell HL-60, whose function may be related with under-regulating genes of perishing survivin and bcl-2.