紫外分光光度法分析测定“黑美人”土豆原花青素
摘要
[Objective] The aim was to determine the content of proanthocyanidins in Heimeiren potatoes and the nutritional value and sanitarian effect through comparing the content of proanthocyanidins with other vegetables in market. [Method] Proanthocyanidins was determinated by ultraviolet spectrophotometry after it was extracted by 75% ethanol at 70 degrees of temperature in water-bath for 30 min, the extration parameters was that 1: 9.14 of the rate of vegetables to 75% ethanol. [Results] The linear region was 3.1-31.0 μg/ml, the regression equation was A=0.003 41+0.008 1C, regression coefficient was 0.998 66.The average recovery ratio (n=3) was between 97.8% and 101.5%, RSD=0.21%. Thus the method was applied to determine proanthocyanidins and obtained a good result. [Conclusion] Heimeiren potatoes are rich in proanthocyanidins, and the content of it was higher than other vegetables in market, it will provide theory support for the extensive use of ”hei mei ren” potatoes in the areas of nutrition and health protection, food and medicine.